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Sequences from the V1 and V6 regions from each phylotype were subsequently extracted and reverse complemented, before being divided into six tiling probes that were printed on an Agilent oligonucleotide array (Agilent Technologies, Palo Alto, CA).
Appended 'rc' indicates the BAC was reverse complemented before alignment.
J774.A1 macrophages were left uninfected or infected with wild-type EPEC, EPECΔ escN, EPECΔ espJ and EPECΔ espJ (pICC32) (complemented), before challenge with C3bi-opsonized RBC.
If the library was constructed with the mate pairs from opposite strands, one strand is reverse complemented before merging so that the entire sequence comes from the same strand in order to better match the generative model described above.
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"There are simple things like being indirect, being sort of muted in your requests, complementing before you ask for something and using the very basic language of politeness" that can lead to more agreement, Keltner said.
But I would have elected to get deeper on the offensive line, or gotten a pass rusher to complement Aaron Schobel, before taking Spiller.
His unbeaten 95, which initially complemented Buttler's 93 before he rallied the remainder of the tail as Plunkett launched one final missile into the night sky, was the highest score by a No8 batsman in one-day cricket, surpassing the 84 made by Kenya's Thomas Odoyo against Bangladesh in Nairobi 10 years ago.
Data were complemented with field notes before qualitative content analysis was applied.
Complemented MEFs were starved overnight before treatment with 30 ng/ml recombinant TNFα for different time points.
And the week before, I ate an incredible Indian meal complemented with Naan bread and a soft drink.
FipA-KO complemented with fipAMTB, was visualized by microscopy before and after subjecting to oxidative stress.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com