The final contigs were concatenated prior to annotation, and the sequence was split and reverse-complemented as necessary to start with the dnaA gene as the first coding sequence.
The transcripts within each of the resulting SCN gene clusters (or orthogroup sets) are reverse-complemented as necessary to ensure identical sequence orientation prior to multiple sequence alignment; the corresponding DNA reference sequence of A. thaliana (or an alternative, user-specified reference) is used to reorient sequences.
For CDS and UTR sequences that spanned multiple fragments, we concatenated FASTA sequences together into a single file and reverse complemented CDS as necessary.
Postoperative CT studies are reviewed interactively on dedicated workstations and complemented with multiplanar reconstructions as necessary, to better depict postoperative anatomy and relevant findings [22, 23, 24, 25].
For single gene loci, the gene was mapped onto its target contig using the scope value, reverse complementing the contig sequence as necessary.
So-called 'soft' policy instruments that respond to the psychological aspects of travel are regularly acknowledged as necessary complements to 'hard' infrastructure investments to effectively promote sustainable travel in cities.
Rather it seeks to entrench and develop political institutions at regional and global level as necessary complements to those at the level of the state".
In case of extramedullary manifestations of leukemia, assessment by imaging, as necessary, was used to complement the blood and bone marrow assessment of response.
26 An alternative system, or one that can be used to complement passive surveillance, is increasingly being identified as necessary to mitigate the harms to patients.
