Exact(1)
We next complemented analysis of the siRNA screens with extensive pathway analyses of the relevant literature, using MetaCore (GeneGo; Thomson Reuters), Ingenuity Pathway Analysis (IPA; Ingenuity Systems), Pathway Studio (Elsevier/Ariadne Genomics) and our own extensive curation.
Similar(59)
Additional analysis of the complemented strain by RT-PCR and sequence analysis showed that the integrated cryptophytic orf222 is transcribed (data not shown).
Growth analysis of the complemented strain Δ::tn7TLACpak in M9 minimal medium with glucose showed intermediate phenotype when compared to the PAO1::tn7TLandand Δ::tn7TLAC strains.
Immunoblot analysis of the complemented mptC mutants expressing either M. marinum or M. tuberculosis mptC resulted in reactivity similar to that of the wildtype, both with regard to LAM migration pattern as well as for Man2/3LAM staining (Fig. 3A C).
Analysis of the fragment ions of the complemented strain demonstrated fragment ions in the n-series with m/ z values of 459.4, 527.3, 595.3, 663.4, and 731.5 (an increase of ∼2 Da in fragments n5 n9).
Moreover, kinetic analysis of the growth (G) isotherms of complemented yeasts, revealed that BjSultr1 1 and BjSultr1 2a/b/c have high affinities for sulfate, as revealed by the kG values similar to the apparent kM values of other plant high-affinity sulfate transporters [ 9, 41- 44] indicating that all the B. juncea clones have retained their functions.
Qualitative thematic analysis of the transcripts and field notes was undertaken using a social constructivist approach and complemented by documentary organizational, programme and policy analysis.
PCR analysis of complemented mutants clearly indicated the presence of the comE1 gene.
TAG content was further analyzed in 9 independent complemented lines of the cw15sta6 mutant.
Complemented strains of the MtΔP27 mutant [ 5] expressing either P27 or P55 were generated in this study.
After the previous step, the complemented path-sets of the product are checked for inclusion between them.
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