The experiments of functional complementation with the complemented strain CΔsalKR showed clearly that the reintroduction of salKR into ΔsalKR restored its high pathogenicity.
Complementation with wild-type nucSTB restored low mutation rates to M. smegmatis ΔnucS.
& Hamra, F.K. Sterile testis complementation with spermatogonial lines restores fertility to DAZL-deficient rats and maximizes donor germline transmission.
Complementation with the AT-hook mutant LEDGF/p75AT- (R183D, K192D and R196D 67 resulted in a similar localization as LEDGF/p75BC (Fig. 4B).
(A) Western blot showing LEDGF/p75 depletion in LEDGF/p75KD cell lines and the complementation with the respective LEDGF/p75 truncations.
As with M. smegmatis, low mutation rates were recovered by complementation with the wild-type S. coelicolor nucS gene (Fig. 2c, Supplementary Table 3).
All phenotypic changes observed could be reversed by genetic complementation, with exception of those seen for the ptb mutant.
Disease occurrence was not due to subversion of Cd28-deficient Tregs into pathogenic cells, as complementation with normal Tregs prevented disease occurrence.
The function of mouse Apollo was determined using Cre-mediated gene deletion, complementation with Apollo mutants, and the TRF2-F120A muthat that cannot bind Apollo.
Subsequent complementation with the wild-type gene and promoter restored growth, demonstrating stable transcription from the broad-host-range plasmid employed.
The approach whether used in complementation with GWAS or as a stand-alone approach could define the genetic architecture of the complex phenotypes.
