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While this is mandatory for method development, complementary, validation against more complex test-cases is essential, in order to prove the method's final scope of capabilities.
By this means, data partitions obtained via different clustering techniques can be continuously refined (via selection and merging) by a population of fusion hierarchies having complementary validation indices as objective functions.
Therefore prediction of interaction complex structure could constitute complementary validation.
In all cases, complementary validation methods should be used (e.g. RT-qPCR) and cell viability and fitness assessed.
Triangulation between the researchers who participated in the data collection and analysis process was used as a complementary validation procedure [ 27, 28].
Additional sequencing was performed for Ba4599 on the 454 GS FLX instrument (454 Life Sciences, Branford, CT, USA) to provide complementary validation of whole genome sequencing SNP calls and improve contiguity of the genome assembly.
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Laribus is supposed to be a complementary certificate validation service that provides an additional layer of security.
These results suggest that the application of GWA approaches using cell-based model systems, when combined with complementary functional validation, can provide insights into mechanisms responsible for variation in cytidine analogue response.
We present two different and complementary promoter validation tools based on transient expression in lima bean cotyledons and production of stably-transformed soybean hairy roots.
Authors' Response: Unless comprehensive complementary experimental validation of high-throughput mass spectrometry studies are undertaken for the two eEF1A isoforms and other proteins it will be difficult to estimate false positive rates.
It can be used for a quick estimation of the modal parameters, or as a complementary approach for validation purposes.
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