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They may be involved in gene expression control by duplex formation of their complementary transcripts, as detected in several other systems [42].
Thereafter, the complementary transcripts have two options.
RISC uses miRNA as a template for recognizing complementary transcripts.
These fragments can bind to complementary transcripts and downregulate their expression via an RNA interference-like mechanism, as explained above.
Seven of the ten tested riboprobes were able to identify complementary transcripts in the total RNA mixture (Fig. 3).
These nat-siRNAs can be incorporated into the RNA-induced silencing complex (RISC) and act to guide the cleavage of complementary transcripts [ 9, 17].
Similar(44)
As the intensity of a probe depends not only on the concentration of its complementary transcript but also on its sequence, such an approach may be problematic.
Nevertheless, these observations provide evidence for the existence of a second naturally occurring grn complementary transcript, namely ASgrna.
Activated RISC then binds to complementary transcript by base pairing interactions between the siRNA antisense strand and the mRNA.
The basic fibroblast growth factor gene (bFGF) has a tail-to-tail complementary transcript encoding GFG, which is a member of the MutT family of antimutator NTPases [ 17].
This study suggests that SSH could be used as an alternative and complementary transcript profiling tool to GeneChip microarrays, especially in identifying novel genes and transcripts of low abundance.
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