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As the intensity of a probe depends not only on the concentration of its complementary transcript but also on its sequence, such an approach may be problematic.
Nevertheless, these observations provide evidence for the existence of a second naturally occurring grn complementary transcript, namely ASgrna.
Activated RISC then binds to complementary transcript by base pairing interactions between the siRNA antisense strand and the mRNA.
The basic fibroblast growth factor gene (bFGF) has a tail-to-tail complementary transcript encoding GFG, which is a member of the MutT family of antimutator NTPases [ 17].
Complementary transcript and network-level analyses of these data, combined with in situ immunohistochemistry of major pattern recognition receptors in the vaginal mucosa, permitted the phenotypic and functional characterization of these populations.
This specific expression pattern is confirmed by examining the signal obtained using sense riboprobes corresponding to grn1 and grn2 that, after prolonged exposure, detect weak signals from their complementary transcript (ASgrn1-2) in the pharyngeal region of these animals.
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They may be involved in gene expression control by duplex formation of their complementary transcripts, as detected in several other systems [42].
Thereafter, the complementary transcripts have two options.
RISC uses miRNA as a template for recognizing complementary transcripts.
These fragments can bind to complementary transcripts and downregulate their expression via an RNA interference-like mechanism, as explained above.
Seven of the ten tested riboprobes were able to identify complementary transcripts in the total RNA mixture (Fig. 3).
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