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The first 12 nt of the 3' leader sequence of APMV-5 are complementary to the last 12 nt sequence of the 5' trailer regions suggesting the presence of conserved elements in the 3' promoter regions of the genome and antigenome.
The double-stranded random DNA library was generated by a primer extension reaction, in which ∼20 μg of the random oligonucleotides was mixed with the reverse PCR primer complementary to the last 18 bases, T4 DNA polymerase (New England Biolabs), and dNTPs in a final volume of 50 μL.
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The second RT-PCR screening approach utilized the primers' pair complementary to the first and to the last exon of Trx.
At the moment this is complementary to the taxi market because last mile and touristic transport aren't always what the taxis are doing.
Reverse transcription primers (44 nt) were designed so that the first 36 nt formed an internal stem loop and the last 8 nt were complementary to the mature miRNA sequence of interest.
The last 20 bp of this primer are complementary to the 3′ end of the pre-BEA PCR product.
Strictly speaking the primer is not specific as only the last 4 - 8 bases in the 3' end are complementary to the miRNA.
To open the hinge, a strand complementary to the fuel strand is put into the solution.
"The brick is also complementary to the brick on the School of Social Work, though not identical.
"Parliament should be complementary to the executive system, not hold it up," he said.
No. Are there people whose activities are complementary to the businesses we are buying?
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CEO of Professional Science Editing for Scientists @ prosciediting.com