Sentence examples for complementary restriction from inspiring English sources

Two complementary restriction derived large-insert BAC libraries of a single A. formosa plant were used for constructing a physical map.

The sequence was engineered with a HindIII restriction site and Kozak sequence (GCCACC) at the 5′ end, and a stop codon and EcoRI restriction site at the 3′ end, and cloned into the expression plasmid pCDNA3.1 (Invitrogen) using the complementary restriction sites.

In order to do this we used two complementary approaches; restriction endonuclease digest analysis of a DXZ4 bacterial artificial chromosome (BAC) clone, and extended DNA fiber fluorescence in situ hybridization (FISH).

For example, if a fish spawning-aggregation site is protected in one jurisdiction, complementary seasonal restrictions on catch of that species in neighbouring jurisdictions can provide increased ecological and fisheries benefits in all jurisdictions.

Digests were run on polyacrylamide gels to separate heteroduplexes of each polarity from one another and from homoduplexes, transferred to membranes, hybridized with a non-radioactively labeled probe complementary to the restriction fragment, and bands visualized with a chemiluminescent substrate and quantified.

Fragments were ligated with adapters complementary to the restriction sites of the enzymes.

Clones were generated by PCR from full-length complementary DNAs and restriction-cloned in the appropriate vector (see also Supplementary file 1 for a complete list of all the constructs).

Fusion TFs were also generated by PCR from full-length complementary DNAs and restriction-cloned in fusion in pUAST or pUASTattB vector with the N-terminal (1 173) fragment of Venus (VN) at the 5′ or 3′ end (see Supplementary file 4).

The recipient expression vector contains two BsaI restriction sites complementary with the restriction sites from the entry clone (Fig 1, plasmid B).

These primers contained complementary sequences for amplifying restriction fragments with ligated adapters, binding PCR products to oligonucleotides that coat the Illumina sequencing flow cell and priming subsequent DNA sequencing reactions [26] (Figure 1).

Each adaptor consists of a pair of oligomers designated S and L. The three adaptors shared a common sequence (bold) and had a cohesive end complementary to the PstI restriction site (underlined).