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Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells.
We also mention some complementary protocols, including the Real Time Streaming Protocol (RTSP) for control of streaming media, and the Wide Area Service Discovery Protocol (WASRV) for location of telephony gateways.
A rapid and general access to ortho-haloaminoarenes has been developed by aryne insertion into N-chloramine, N-bromoamine, and N-iodoamine bonds via two complementary protocols harnessing fluoride-promoted 1,2-elimination of ortho-trimethylsilyl aryltriflates.
On the other hand, these techniques can be considered as complementary protocols to affinity-based techniques, and can broaden the phosphoproteomic coverage (Bodenmiller et al. 2007).
The lists of cases collected through these complementary protocols by GOVS and the Central Laboratory for Quality Control of Poultry Production was provided by the Emergency Centre for Transboundary Animal Diseases Unit of the Food and Agriculture Organization in Egypt.
The two complementary protocols generated a list of metabolites changed by UV-B in irradiated leaves and either translocated to shielded leaves or elevated there by an unknown signal.
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A complementary protocol to TCP, though it makes no guarantees about data delivery or reliability.
Projet Lac uses standardized fish population surveys, following the standardized European protocol (Comité Européen de Normalisation 2005) and a locally developed complementary protocol that pays special attention to deep sections of the lakes that are not well sampled by the European protocol (Alexander et al. 2014).
By using two complementary enrichment protocols (hydrazide chemistry and lectin resin) and two orbitrap-based and triple TOF-based double high LC-MS/MS systems, we have achieved an in-depth identification of 25 N-glycoproteins that mapped on to 53 sites on RJ proteins.
A complementary enrichment protocol was adapted from the existing standard ISO 6579 2004 and consisted of enrichment in Buffered Peptone Water (BPW) 22 ± 2 h and a second selective enrichment for 6 h in Rappaport Vassiliadis with Soya (RVS).
To do so with an appropriate level of safety, it is recommended that these alternative treatments be attempted as part of a complementary treatment protocol.
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