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No BiFC was obtained between a fusion construct and the complementary isolated VC or VN fragment, highlighting that the interaction between NvPbx and NvMeis fusion proteins was not artificially induced by the inherent affinity of the VN and VC fragments.
This is now more clearly stated in the Result section: "No BiFC was obtained between a fusion construct and the complementary isolated VC or VN fragment, highlighting that the interaction between NvPbx and NvMeis fusion proteins was not artificially induced by the inherent affinity of the VN and VC fragments".
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Complementary investigations isolated a carboxyl/aspartate protease from activated macrophages utilizing pepstatin-A affinity chromatography.
A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially.
As a complementary approach, we isolated endothelial cells from Pkd1endo− (n = 5) and Pkd2endo− (n = 4) embryos as well as controls and assayed for deletion using either a PCR based strategy or Western analysis (Figure 7E, F, G, H).
These constructs were introduced into uracil auxotroph strains, and genetically complementary transformants were isolated successfully on minimal agar plates.
Furthermore, we argue that both integration strategies should be seen as complementary, rather than isolated strategies as there is an anticipated causal effect between both.
Although these complementary endonucleases were isolated from the same Streptococcus pneumoniae species, they do not co-exist in the same genome, probably because of their endonuclease activities.
Total ribonucleic acid was extracted, messenger ribonucleic acid was isolated, and complementary deoxyribonucleic acid was synthesized.
CD40 complementary DNA clones were isolated from human, cynomolgus monkey and rabbit tissues.
From isolated RNA, complementary DNA was prepared and used for expression analysis [ 18]. 16S rRNA was used as an internal control.
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