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Briefly, cells were lysed in 20 μL cell lysis buffer and treated with 5 μL NADPH/NADH/NADP+/NAD+ extraction solution for 15 min at room temperature before being neutralised by an equal volume of complementary extraction solutions.
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In this context, we propose that a complementary hydroxylapatite extraction assay (EI assessment) should be added to the usual tests to identify ER-positive tumours.
2. URL feature extraction: complementary attributes such as website text and HTML tags are extracted from the raw data for each URL provided.
RNA extraction, complementary DNA (cDNA) synthesis, and real time PCR were performed with primers designed for amplification of HTLV-1 tax cDNA, and human housekeeping gene hypoxanthine ribosyl transferase (hprt) for internal calibration [22].
Total RNA extraction, complementary DNA (cDNA) synthesis, and qPCR were performed as described previously [ 12].
RNA extraction, complementary DNA synthesis and quantitative real-time PCR analysis using SYBR® Green were performed in triplicates as described (Lyck et al., 2009).
RNA extraction, complementary DNA synthesis, Taqman real-time quantitative polymerase chain reaction and Δ Ct analysis were performed according to previous recommendations (Benn et al., 2008).
RNA extraction and complementary DNA ('cDNA') synthesis: Total RNA was extracted from each group (tube) of 15 larvae according to the Trizol-based protocol of Fiumera et al [ 44].
RNA extraction and complementary DNA (cDNA) synthesis were conducted from all tissues.
RNA extraction and complementary DNA (cDNA) synthesis were performed for all samples.
In short, discriminant multi-feature extraction with complementary nature helps to improve the accuracy of human re-id.
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