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Authors' Response: Unless comprehensive complementary experimental validation of high-throughput mass spectrometry studies are undertaken for the two eEF1A isoforms and other proteins it will be difficult to estimate false positive rates.
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As such, there is a great need for further experimental validation or complementary annotation approaches for conventional genome annotation.
Therefore, the under-replicated screens can only be conducted when followed by a rigorous experimental validation with complementary technologies and adequate sample size.
Using two complementary methods, aCGH and qPCR, we performed experimental validation to confirm individual copy number variants.
For the experimental validation, 81 randomly selected DEGs were validated.
The inclusion of multiple ab initio gene-finders gives rise to complementary predictions by providing alternative models that can be used for experimental validation and may lead to the determination of true gene structures.
A complementary set of design tools has been developed to aid researchers with nuclease design, target site selection, and experimental validation.
The predictions of the hypothesis, in particular, the nuclease activity catalyzed by PAZ-domain-containing but not by PAZ-domain-lacking pAgos, the complementary activities of associated putative nucleases, and guide DNA or RNA binding by the APAZ domains are amenable to straightforward experimental validation.
"This is the most definitive experimental validation of these predictions to date," he said.
Experimental validation is provided.
Experimental validation has not yet been completed.
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