Your English writing platform
Free sign upExact(6)
Pairs with complementary ECHO probes exhibited hybridization-sensitive fluorescence emission for the mRNA expressed in a living cell.
We next prepared these tag RNA sequences and the complementary ECHO probes (D514 or D640, Figure 1), and confirmed the sensitivity and orthogonality of the fluorescence emission.
The pairs of an 18-nt RNA tag and the complementary ECHO probes are highly thermostable, sequence-specifically emissive, and orthogonal to each other.
This is a new method for spatiotemporal monitoring of RNAs in cells by using pairs of the repeated 18-nt tags incorporated into the mRNA 3'-UTR and the complementary ECHO probes.
Three pairs of an 18-nt RNA tag and the complementary ECHO probes in this study are highly thermostable (Tm approximately 70°C), sequence-specifically emissive, and orthogonal to each other.
The requirements for effective tag sequences are (i) sequence length appropriate for higher duplex stability but smaller tag size for synthesis of compact repeated tags; (ii) no formation of higher-ordered structures; (iii) no interference to the sequence, structure, and function of RNA main parts; and (iv) avoidance of self-dimerization of complementary ECHO probes.
Similar(53)
The additional N N additions for the HGM submodel stem from the fact that the HGM submodel's output has to be added to the complementary-part echo estimate obtained with the HM (recall (35)).
which is combined with the complementary-region partitions' echo estimate of the HM submodel according to begin{aligned} hat{mathbf{y}}_{text{SA}}{ kappa)} & = mathbf{W}_{01}mathbf{F}^{{mathrm{H}}} left(sum_{b=1}^{{B}}underline{mathbf{x}}_{b}{ kappa-{p_{mathrm{d}}})}odothat{underline{mathbf{h}}}_{b}{ kappa-{p_{mathrm{dkappa-1)} right.
The support at the Summit for breastfeeding and complementary feeding was echoed at the Special Sesson on Children 11 years later, with a call to "protect, promote and support exclusive breastfeeding of infants for 6 months and continued breastfeeding with safe, appropriate and adequate complementary feeding up to two years or beyond.
The complementary premium car mount echoes the design of the 8100T, plus it has additional functionality too.
What Rebecca and I found in Havana, and amplified considerably while working in Rochester, was that two distinct yet complementary photographic visions could visually echo the many layers of a city.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com