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The way in which DCD procedures are described varies, from no specific protocol (two countries) to detailed protocols with complementary consensus statements and ethical frameworks, as in the UK [ 17].
For the CREB promoter, the two complementary consensus oligonucleotides were: 5′-AGAGATTGCCTGACGTCAGAGAGCTAG-3′, 5′-CTAGCTCTTGACGTCAGGCAATCTCT-3′, and the two complementary mutant oligonucleotides were: 5′-AGAGATTGCCTG TGGTCAGAGAGCTAG-3′, 5′-CTAGCTCT CTGACCACAGGCAATCTCT-3′. Oligonucleotides 3′- were labeled by biotin.
The first 8 to 13 nucleotide bases at the 3' ends of all three RNA segments have a sequence that is conserved in the viruses of this genus, with a complementary consensus sequence occurring at the 5' end; the ends of the segments are noncovalently linked so that the RNA occurs in a loosely bound circular configuration within the nucleocapsids (11 ).
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We posit that another six areas of complementary ethical consensus and emphasis have either emerged or moved to centre stage.
The complementary benefits of consensus and T/F Env immunogens raise the possibility that vaccines utilizing a combination of consensus and T/F Envs may be able to induce neutralizing responses with greater breadth and potency than single Env immunogens.
The sequences complementary to these consensus target sequences (corresponding to the theoretical guide strands of CODEMIRs) were evaluated for binding to the target mRNAs using RNAhybrid software [ 15].
The combined pool of indexed gDNA fragments was enriched for Ves insertions by binding a biotinylated probe that is complementary to the consensus Ves3ML subfamily element (fig. 1 C).
Detection of SVs that are >5 kb in size was performed using two complementary approaches, a consensus-based approach and a single-molecule approach.
The F sequence consists of 6 12 bases that are complementary to a target consensus sequence.
An oligo-dT primer harboring a consensus sequence (complementary to the reverse primer) was used for reverse transcription reaction.
This interaction is mediated by the 5′-free end of U1snRNA, which has a 9 base-long complementary sequence to the consensus (Mount et al, 1983; Sharp, 1994; Black, 1995).
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