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Extracted RNA was subjected to complementary cDNA synthesis using RT2 preamp cDNA synthesis Kit Catt # 330401) according to the manufacturer's instructions.
HA-PRR7 was generated by inserting the HA epitope at a position 15 amino acid upstream of the C terminus of human PRR7 complementary (cDNA) to avoid the disruption of its interaction with PDZ-domain-containing proteins.
Extraction of total RNA and synthesis of complementary cDNA were performed as described [46].
The complementary cDNA strands were annealed by heating to 95°C and cooling slowly to 72°C.
Microarray technology relies on the hybridization of a labeled target sequence to a complementary cDNA or oligonucleotide probe immobilized on a glass surface.
Complementary cDNA was synthesized from 1 µg of total RNA at 42°C for 30 min using MMLV reverse transcriptase, Rnasin, 4mM dNTPS, and 1.2 µg/ml random hexanucleotides (all reagents from Promega).
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RNA from GMPs, MDPs, and CDPs was also processed into complementary DNA (cDNA) using qScript cDNA SuperMix (Quantabio).
Angiotensin-converting enzyme 2 (ACE2) was firstly identified from human cardiac left ventricle complementary DNA (cDNA) library and lymphoma cDNA library by two separate groups [7, 8].
complementary DNA (cDNA) was synthesized using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher) according to the manufacturer's protocol.
Complementary DNA (cDNA) was made using High Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Foster City, CA).
Complementary DNA (cDNA) was synthesized using qScript™ cDNA SuperMix (Quanta BioSciences).
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