Deep sequencing is a complementary approach capable of detecting viruses that are too divergent from known viruses to be detected by either PCR or microarray techniques [16], [17], [18], [19], [20], [21], [22]; reviewed in [6].
All the primers included 9-bp non complementary extensions capable of generating KpnI or HindIII restriction sites.
A band was amplified when complementary sequences capable of primer binding were present in genome on opposite DNA strands within a PCR amplifiable distance.
In addition, to minimize environmental impact, a complementary process capable of converting the generated by-products present in the effluent, thereby reducing the chemical oxygen demand, is an absolute requirement.
Recent results suggest an additional form of regulation, whereby overlapping genes in complementary strands are capable of reciprocal regulation through formation of complementary anti-sense transcripts in prokaryotes [ 5] and also somewhat surprisingly in eukaryotes [ 7] and vertebrates [ 21].
Upon transient transfection of the shRNA libraries into the selection cell line clones indicated by asterisks in Fig. 2c, only the complementary library is capable of downregulating the HSV-TK expression significantly as compared to the non-matching libraries (Fig. 2d).
Long-term competitiveness, however, depends on whether the innovator builds a strong position in specialised complementary assets and is capable of reconfiguring them over time in line with changes in the market environment.
The multivariate zoning that was presented focuses on the effect of the groundwater processes in the water table, whereas the complementary methods may be capable of providing an improved differentiation of the role of these processes (recharge, discharge, leakage, river interaction, pumping) in the aquifer.
These two spacecraft are capable of revealing complementary aspects of the Venusian atmosphere.
One of which is to distribute smart TVs as a content platform capable of providing complementary programming, t-commerce, and real-time analytics.
