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Liu, Q., Wang, F. & Axtell, M. J. Analysis of complementarity requirements for plant microRNA targeting using a Nicotiana benthamiana quantitative transient assay.
Noticeably, the U1F9wt that differs for one mismatch only from the mutant-specific U1snRNAs was ineffective on F9 exon 2 5′ss mutants, thus strengthening the notion that the complementarity requirements between the U1snRNAs 5′ tail and the 5′ss for a functional splicing outcome are far from being known (21).
This has been shown to be important to accurately estimate the affinity of small fragments, which by having a limited number of interactions with the protein, and less shape complementarity requirements as compared to drug-like molecules, may adopt multiple binding modes with similar thermodynamic stability.
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We shall later show that G can be modified to satisfy the reverse complementarity matching requirement without losing optimality.
Balance and complementarity is required in both the batting order and the bowling unit.
c Complementarity, Exophora Essential d Complementarity, Agent-Object Essential.
Through increasing the connectivity of different forms of energy, analyzing their complementarity, and coordinating energy requirements of the user, an MIES can realize security, energy savings, high efficiency, low carbon emissions, cascade utilization and environmental protection.
This criterion is consistent with other works in which the requirement of complementarity in tRNAs and aminoacyl-tRNA-synthetases renders almost perfect symmetry of the table of the genetic code (e. g. [25] [26]).
To eliminate the potential misleading influence of these sequences, which may be under selection due to the requirement for complementarity between miRNAs and their targets, on the similarity test, each foldback arm with hits (E<0.05) in the FASTA search was analyzed independently with and without the miRNA or miRNA-complementary sequences.
However, the requirement for complementarity of only seven or eight nucleotides could result in hundreds of possible targets (Lim et al, 2005).
Each CRISPR spacer, however, is in principle constrained to target a specific phage because of the requirement for complementarity to the entire RNA sequence (though see below on the possibility of CRISPR seed sequence).
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