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To test whether the destruction of the antisense complementarity alone (without structural change) abolishes regulation by Yfr1, we constructed another Yfr1 mutant.
Furthermore, the ability of miRNAs to target mRNAs with only partial complementarity [ 12] indicates that in many cases identification of miRNA targets may not be possible based on seed match complementarity alone [ 10].
Analogous to our target prediction analyses with the novel transcripts described above, we were unable to perform a more comprehensive (based on more complex RNA-RNA interactions rather than simple complementarity alone) analysis of potential leishmania tsRNA-host target mRNA interactions due to the lack of appropriate tools for large datasets.
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Thus, the combination of complementarity and accessibility alone resulted in interactions with an implicit seed.
Unlike previous methods that weight miRNA-target interactions based on sequence complementarity or gene expression correlation alone, we estimate the overall influence of a miRNA on its target based on the target gene expression level and the gene expression levels of the interaction neighborhood of the target.
As when prioritizing based on species richness or endemism alone, the objective when using complementarity is to maximize the biodiversity benefit per dollar invested.
A recent meta-analysis of mainly plant biodiversity experiments found that, in most studies, the relationships between biodiversity and ecosystem processes were driven by a combination of selection effects and complementarity effects, rather than by one mechanism alone [11].
This occurred even when using the in silico filter for strand invasion artifacts developed in [ 9], indicating that the presence of "C -rich regions alone in mRNA without additional C -richentaregions the TS oligo malone suffinient for promRNAng strand invasion.
We highlight the complementarity of replication and rejuvenation, showing improvements of resilience not attainable with any of the techniques alone, but possible when they are combined.
Theory of complementarities: This theory developed by Gargallo-Castel and Galve-Górriz (2007) highlights the fact that ICT alone is not sufficient to guarantee increase in organizational output.
Moreover, this complementarity is successfully summarized using our OPLS-DA-based approach, which shows an overall higher enrichment than Pearson alone.
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