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There is an emerging need for viral gene specific quantitative PCR (qPCR) assays that validate and complement whole transcriptome level technologies, including microarray and next generation sequencing.
To complement whole genome approaches, particularly for complex diseases where effect sizes are likely to be small, locus specific methods are also required for investigating multiallelic CNV loci.
Our study demonstrates that careful and rigorous primer optimization and an examination of sequence variation among families or individuals is a critical step before real time PCR assays are used to complement whole transcriptome analyses, especially when dealing with short fragments such as those generated by differential display techniques.
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Transcriptome sequencing therefore complements whole or partial genome sequencing approaches.
Every section has to stand or fall on its own merits, as well as complement the whole.
Ideally the tie will complement the whole ensemble, so it should be matched with the shirt as well as the suit.
She was the only person I knew who had one... and had her ringtone set to Abba's "Take a Chance on Me" to complement her whole retro non-techie vibe.
This prompted us to test potential killing of the leptospiral strains by the different cell types, complement, or whole blood.
During the time frame for submission of revisions, we were not able to collect enough Neurog1 CreERtdTomato-positiveitissueissue to complement the whole mount preparations done with WT tissue.
Thus, all vertebrate GATA genes are located within extensive paralogons providing strong support for an origin of the vertebrate GATA gene complement by whole genome duplication events from two ancestral GATA loci, one GATA123 gene and one GATA456 gene.
In non-model species with large genome size, EST (expressed sequence tag) sequencing and their annotation can provide the first step towards understanding the transcriptome and expression patterns of specific genes, which can complement the whole genome sequencing, and can assist with genome sequence annotation.
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