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It should be noted that calculation of the number of subclasses in major classes takes into account both direct and inverse complement of a sequence in the proximal-8 region to eliminate those subclasses that seem to represent two different sequences but in fact are direct and inverse complement versions of the same sequence.
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A classification-type HMM with circular local alignment and a reverse complement version was generated with this data (Figure 2A and File S1).
Automated annotation was performed by BLASTp and BLASTx searches (e-value < e-10) against the S. lycopersicum (version iTAG 2.3 proteins), S. tuberosum (version 3.4), A. thaliana protein complement (version TAIR 10 pep) and the UniProtKB/Swiss-Prot database (release 2012_02).
The reverse complement sequences of the converted reads are needed because the reference genome only contains the forward strand; therefore, we need the reverse complement version of the read in order to align it to the reference genome assuming that it matches the reverse strand.
Smith sees ecological and constructivist rationality as two complementing versions of economic rationality, while Gigerenzer regards the study of fast-and-frugal heuristics as the appropriate scientific method for exploring real-world rationality.
Thus, a vaccine consisting of defective, complementing versions of the FMDV genome can evoke both an antibody and a cellular immune response, which results in protection of swine when challenged with the corresponding virulent FMDV.
The experience with violence and aggressive behaviour and the measures used for managing such situations were recorded with a self-administered questionnaire, which is a modified and complemented version of the Staff Observation Scale Revised (SOAS-R) by Nijman and colleagues of the year 1999 [ 15].
Efforts to complement with versions of MORN1 missing one or more MORN domains failed, indicating that all MORN domains are required for function.
The crucial decision was adding a second edition of the hit singing competition, "The Voice," on Monday to complement the version that ran in the spring.
Was it perhaps seen as a complement to other versions of Reynard, for grown-ups, in the library, or as a notable adoption of Caxton's early English version?
The neo-country outlaw singer-songwriter has crafted a bittersweet original country waltz, "South Alabam Christmas," to complement his easygoing versions of the inescapable yuletide duet "Baby It's Cold Outside" (with Hawaiian singer Lily Meola), "Mele Kalikimaka" (a lively collaboration with the Secret Sisters), Willie Nelson's "Pretty Paper" and Mel Tormé's "The Christmas Song".
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