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In addition, one of PWMs can undergo reverse complement transformation.
Two TFBS models can be aligned by PWM shifting and possible reverse complement transformation.
random model and the background probabilities comply with the conditions p(A) = p(T), p(C) = p(G), then the reverse complement transformation of PWM M does not change the score distribution and hence the P-values.
Reverse complement transformation of PWM M is a new PWM M ˜, for which the following relations are valid for any column i: M ˜ A, i = M T, m − i + 1 ; M ˜ T, i = M A, m − i + 1 ; M ˜ C, i = M G, m − i + 1 ; M ˜ G, i = M C, m − i + 1. Reverse complement transformation of a PWM is a PWM that locates the same set of TFBS but on the opposite strand of a DNA heteroduplex.
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The mutant phenotype was complemented by transformation with the wild-type gene.
Free energy landscape analysis further complemented the transformation analysis of peptide conformations.
Furthermore, the albino phenotype of the alb2 mutant was also complemented by transformation with pAUR-PksP (data not shown).
The pabaB22 mutation of strain MAD3682 (Table 1) was complemented in transformation using a wild type version of the AN10991 coding region.
The growth deficiency of C. glutamicum IB2103 in CGXII minimal medium was complemented by transformation with plasmid pIB4000 carrying the cloned dtxR gene.
The mutation was complemented by transformation of the Ames 35 ΔAtxA strain with plasmid pIU68 [ 14], which contains the atxA gene and its endogenous promoter region.
Furthermore, we aimed to elucidate the role of these two borrelial proteins towards complement resistance by transformation of a serum-sensitive B. garinii strain which lacks CFH-binding CRASP proteins.
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