Sentence examples for complement the knockout from inspiring English sources

In contrast to previous observations, all enzymes tested could partially complement the knockout mutation when the genes were weakly expressed.

Sec61β homologs from Saccharomyces cerevisiae and human complement the knockout of sbh1+ in S. pombe.

Collectively, these results indicate that the tail anchor of Sec61β is sufficient to complement the knockout of the entire protein, and that this can be indistinctively accomplished by homologous transmembrane domains ranging from yeast to human.

This flexibility is demonstrated by our use of the system to: 1) knockout a specific operon and then complement the knockout by allelic exchange back at the site of deletion.

To complement the knockout mutants, full-length rv1219c (raaSMtb) was cloned into SpeI and HpaI sites of an integrating vector, pRBexint, to ensure constitutive expression from a dnaK promoter (28).

However, whenever we attempted to complement the knockouts we would discover that differential expression of motility genes was not affected by complementation.

Here we demonstrate that this functional complementation is possible even with higher eukaryotes, as the human homolog of Sec61β also complements the knockout in S. pombe and S. cerevisiae.

Remarkably, the TMD of Sec61β from S. cerevisiae and human also complement the gene knockouts in both yeasts.

Nevertheless, the Sec61β homologs from S. pombe, S. cerevisiae and human complement the phenotypes of knockout strains of both fission yeast and budding yeast, to a level comparable to that of the endogenous proteins.

Although the structure of SA (2-HBA) is very similar to that of DF (3-HBA), SA failed to complement the Xcc xanB2 knockout mutant [ 27, 23].

In addition, heterologous expression of E. coli AdhE or other ADH homologs failed to complement the adhE genomic knockout in terms of restoring 1-propanol production, potentially due to unknown perturbations in the metabolite pool or gene regulation.