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G418R cell lines were screened for their ability to complement the growth of ts4149, a vaccinia virus mutant that harbors a temperature-sensitive mutation in the udg (D4R) gene, at the non-permissive temperature of 39.5°C [51], [53].
The ΔH28ΔH76 mutant was verified to lack superoxide dismutase (SOD) activity by its inability to complement the growth of the SOD double-negative (sodA-, sodB-) E. coli strain CK9C1891 [82].
MVA-permissive DF-1 cells (Fig. 1) were transfected with a udg-expression plasmid pCANudg (see materials and methods) and G418-resistant cell clones were screened for their ability to complement the growth of ts4149, a vaccinia virus mutant that harbors a temperature sensitive mutation in the udg (D4R) gene, at the non-permissive temperature of 39.5°C (not shown).
These include: MVA growth comparable to that achieved with primary CEF cultures, the ability to plaque MVA on DF-1 cells, and the ability to generate DF-1-derived cell lines that stably express poxvirus genes and complement the growth of essential-gene deletion MVA mutants.
All of these proteins are able to complement the growth of the yeast strain ZHY3.
All of these proteins are able to complement the growth of the yeast strain ZHY3 or DEY1453, which is sensitive to Zn or Fe deprivation due to the mutation in both high and low Zn or Fe affinity system.
Similar(53)
We found that InvMce1 complemented the growth of Saccharomyces cerevisiae cet1Δ and ceg1Δ strains in which the endogenous yeast triphosphatase and guanylyltransferase genes were deleted.
Similar to the Arabidopsis ZIP proteins, the grape ZIP protein encoded by the cDNA of VvZIP3 complemented the growth of this yeast mutant.
We found that Magmas is an ortholog of yeast Pam16 and has similar functions; and it complements the growth of yeast cells deleted for Pam16.
Human ISCA is likely an alternative scaffold, since it complements the growth of the yeast ISA1 mutant, which has been demonstrated to function in Fe−S cluster assembly in yeast (88).
However, it is significant that RecBCDEc enzyme can complement the growth defects of LCBD (ΔrecBCD) strain of P. syringae at 4°C, a temperature in which E. coli does not grow.
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