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The ability of the individual constructs to complement the essential NOC4 function was tested by counterselection against the NOC4/URA3 plasmid on 5-FOA-plates.
We screened then for alleles that do not complement the essential functions of rpS5 but partially suppress the rpS5 depletion phenotype.
Growth on 5-FOA plates indicates that the mutant human cDNA is able to complement the essential gene.
In addition, the recently published DOH-WHO Guidelines on Intrapartum and Immediate Postpartum Care, developed together with the Philippine Obstetrical and Gynecological Society (POGS), aims to complement the essential newborn care (ENC) protocol guidelines, to ensure safer and more effective labor procedures.
(D ) Left: HA-tagged WT and mutant rsp5 alleles housed on low copy plasmids were assessed for their ability to complement the essential function of RSP5 in either serially diluted rsp5-1 temperature-sensitive cells grown at restrictive 37°C or in rsp5Δ null cells after eviction of wild-type RSP5 plasmid on 5-FOA.
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A Morgan Schneiderlin-esque addition would be welcome to complement the essentials Coquelin brings.
Fuss is the conduit of a gospel audience's fervor, and it complemented the essential serenity of the baritone Mr. Fountain, the tenor Jimmy Carter and the bass George Scott.
This work is concerned with obtaining bounds on the number of eigenvalues of L in subsets of the complement of the essential spectrum of L0, in terms of the approximation numbers of the perturbing operator K. Our results can be considered as wide generalizations of classical results on the distribution of eigenvalues of compact operators, which correspond to the case L0="0.
According to Taubenberger, this suggests a new strategy for surveillance, one that would include identifying and isolating a local variant of the virus on the verge of acquiring a complete complement of the essential mutations, after which point it would become impossible to contain.
But pedagogical tools, digital or otherwise, are only effective if understood and used as a complement to the essential elements of learning, which is not in the technology itself.
Apparently, although efficiently expressed (Fig. 2B), the mutant allele is not able to fully complement all the essential functions of RPS5.
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