Most importantly, heterologous expression of the respective genes in cells of a S. cerevisiae mutant defective in TAG synthesis complements the defect of the host cells.
Interestingly, depletion of PML can partially complement the defects of an ICP0-null mutant virus [33].
Thus, gas4p is able to fully complement the defects of gas1Δ cells.
Surprisingly, E. coli recBCD or recBC alone on plasmid could complement the defects of the ΔrecCBD strain of P. syringae.
However, the ability of RecBCEc enzyme (lacking RecD), unlike the RecBCPs, to complement the defects of LCBD strain was a little surprising.
The TMDs of the other Sec61β homologs tested were also able to fully complement the defects of the Δsbh1 strain, indicating that the fundamental function of the TMD is conserved between species (Figure 5A C).
The reciprocal ability of RecBCDPs and RecBCDEc to complement the defects of ΔrecBCD strains of P. syringae and E. coli suggests that the enzyme complex as a whole have retained all conserved biochemical activities that are needed for DNA repair and recombination.
When expressed in U. maydis, it can complement the defects of the kpp2 mutant in mating and plant infection [ 56].
The ability of the RecA-GFP chimera to complement the defects of the Δ recA strain in growth, viability, SOS induction, UV sensibility, genetic recombination and swarming motility was compared to that of the RecA-Htg variant.
POR2 encoding VDAC-2 was subsequently identified in a screen for genes which, upon overexpression, could functionally complement the defects of POR1-deleted strains, although its pore forming ability has been questioned [19,29].
