An individual's DNA, chopped into suitable fragments and tagged with fluorescent labels, could then be washed over such a chip, his or her genetic complement read off, and the risk factors for relevant diseases calculated.
We observed 19,655 1D template reads, 9,584 1D complement reads and 7,540 2D reads.
The first run (R7) produced 43,656 template reads (272 Mb) and 23,338 complement reads (125 Mb).
Complement reads may be present if a hairpin has been successfully ligated.
Template and complement reads correspond to sequencing of the two DNA strands.
Metrichor™ combines template and complement reads to produce a consensus (2D) [ 9].
When both 1D template and 1D complement reads are basecalled, a 2D consensus sequence is determined based on complementarity.
The decision to reverse complement reads is made for all reads at the beginning of compression before any reads are encoded and before the read starts are encoded.
After the sequencing process, we obtained 3404 reads, of which 58.5 % were template reads (1991), 23.8 % were complement reads (812), and 17.7 % were 2d reads (601).
Depending on the type of read library being compressed, we also take advantage of the ability to reverse complement reads to gain better compression.
