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The complement reaction either can trigger the lysis (bursting) of the invading microbe or can attract microbe-killing scavenger cells that ingest, or phagocytose, the invader.
After termination of complement reaction both untreated and sialidase-treated PA in TSB and TSB containing Sia were washed, diluted and appropriate dilutions were plated on TSB-agar for bactericidal assay.
Results of CuAAC reactions with a minor groove-localized alkyne ± a DNA complement; reaction results with various azides and RNA containing 7-EAA or 5-EU; detailed method for the purification of RNA; and X-ray crystallography data collection, phasing, and refinement statistics.
The outcome, that is, the intensity of the complement reaction, is categorized through the percentaged indication of dead (red colored) cells using a score system of the National Institute of Health (Washington, USA) as shown: score 1: <10% (negative), score 2: 10 20% (doubtfully positive), score 4: 20 50% (weakly positive), score 6: 50 80% (positive), and score 8: 80–100% (strongly positive).
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Initial and follow-up serum specimens were tested for lymphocytic choriomeningitis virus by complement binding reaction.
The tests used were indirect immunofluorescence antibody titres (IFAT), complement fixation reaction (CFR), and enzyme-linked immunosorbent assay (ELISA).
Diagnostics of bacterial pathogens included ELISA, Western blot, and PCR for Borrelia burgdorferi sensu lato and Treponoma pallidum, complement binding reaction for Leptospira spp., and standard microbiologic culture methods.
To guarantee high propylene yield, a good catalyst must have low hydrogen transfer, high accessibility and prevailing matrix technology to complement the right reaction system and reaction conditions.
mAb against Class I and Class II oncoantigens not only functionally inhibit the activity of their targets and recruit antibody-dependent killer mechanisms and complement-dependent reactions but also recruit the host adaptive response and act to some extent as a vaccine [ 84, 85].
This comes as a complement of the reaction layer theory known for its performances and also for its limits precisely for slow kinetics.
KEGG analysis indicated that the differential proteins participated in the complement clotting chain reaction (Additional file 3).
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