This experimental design was chosen because most of the COMP-specific mAbs are of the poorly complement-fixing IgG1 isotype, and complement activation is one of the critical factors for development of arthritis [ 25, 26].
Previously, it was shown that OpuAC could complement OpuAΔSBD, albeit poorly due to the low affinity of OpuAC for the membrane-domain of OpuA [14].
The noted signal at this temperature was likely due to leaky expression of D:: eGFP from the plasmid, but more importantly demonstrates the strong ability of gpD::eGFP to complement for the poorly functional gpDQ68Y protein.
As pFL80 poorly complemented trp1 mutant strains, which overexpressed the pURA2 promoter (due to some transcriptional interference between LacZ and TRP1), we also used pFL80-H2, a pURA2∷LacZ reporter with a lower LacZ expression.
Any investor who has his performance measured against a global bond-market index, whose weighting is ever-more skewed towards the Japanese market, will have done very poorly without a full complement of JGBs.
However, the diversity and complement of longins in eukaryotes is poorly understood.
nrdHIEF however, is poorly expressed and can not complement an nrdAB conditional mutant unless a second nrdHIEF copy is provided, either as a merodiploid or in a plasmid-based copy [16].
However, the immune cell complement of human and rodent mammary gland is poorly defined.
The functions of FHR-1 and other factor H-related proteins are poorly understood, but appear to include control of complement activation, cellular adherence and other functions [13], [41] [45].
Although the mechanisms for these types of treatment are poorly understood, they can be attempted as a complement to pharmacological treatment.
