The full complement of targets found in each of the five degradome libraries is presented in Additional file 1.
The main aim of targeted application of complement inhibitors is to reduce any potential side effects whilst trying to treat a condition [66].
It has underlined the importance of complementing targeted health interventions with integrated approaches which strengthen healthcare systems overall," the report said.
For consistency, in this manuscript we used the term "target" for any predicted mRNA gene of some known miRNA, accordingly "non-target" is used for the complement of predicted targets.
With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently atypical hemolytic uremic syndrome (aHUS), the viability of targeting the complement system was demonstrated.
In theory, the technique is simple yet elegant: the complement of a target sequence of messenger ribonucleic acid (mRNA) encoding the protein of interest is used to disrupt translation or prevent translation initiation.
We give an example of a deviation from the expected thermodynamical behavior for the probe set 1091_at due to annotation problems, i.e., the surface-bound probe is not the exact complement of the target RNA sequence, because of errors present in public databases at the time when the array was designed.
Currently, 21mers from the reverse complement of the target transcript(s) are considered effective amiRNA candidates, if they have an "A" (sometimes also "U") at position 10 and display 5' instability (higher AU content at the 5' end and higher GC content at the 3' end).
Otherwise, the reverse complement of the target sequence was checked, and hits were reverted.
Although this complement of WNK8 target genes (which is based on altered transcript levels in the three mutants) is more stringently defined than the complement of the EDM2 target genes (which is only based on altered transcript levels in one mutant), the former is almost twice as large as the latter, suggesting that WNK8 may have a wider spectrum of biological roles than EDM2.
