According to this model, complementary partial 'loss-of-function' mutations in both genes results in the sharing of a full complement of functions that had been performed originally by the single ancestral gene [ 2].
Deficiency Df 2R w45-30n lacks regions 45A-45E, theremovingoving the pkn gene and fails to complement loss-of-function pkn alleles such as pkn 06736, pkn, and pkn.
Our novel findings also complement gain of function studies in cell cultures and transgenic mice by demonstrating that Notch signaling participates in late-stage granular layer differentiation.
We made use of this strategy to disrupt the endogenous Pfama1 gene in W2mef while simultaneously complementing loss-of-function with the antigenically distinct 3D7 Pfallelellele (Fig. 1).
Thus, future studies of mir-35 family function may have broad implications for our understanding of the reversibility of microRNA target repression and the full complement of microRNA functions during development.
In addition, xen72−3 failed to complement a loss-of-function allele of Asx (AsxXF23, [16], [17]), suggesting that xen72−3 is an allele of Asx.
To complement the loss-of-function studies, we examined the effect of overexpressing PTK6 in both MCF-10A cells and immortalized human mammary epithelial cells (HMLE).
All but ATPalpha CJ7 failed to complement both loss-of-function alleles ATPalpha DTS1R1 and ATPalpha DTS2R3 for lethality.
This refers to the ability of a human gene (or set of genes) to complement the loss-of-function phenotype of its yeast ortholog (or set of orthologs).
Unlike VvMYB30, VvMYB60 was able to complement the loss-of-function atmyb60-1 mutant, indicating that VvMYB60 is the only true ortholog of AtMYB60 in the grape genome.
To complement these loss-of-function approaches, we used an activation-tagging screen to identify gain-of-function downstream components that may act in a redundant manner (Weigel et al. 2000).
