Deficiency Df 2R w45-30n lacks regions 45A-45E, theremovingoving the pkn gene and fails to complement loss-of-function pkn alleles such as pkn 06736, pkn, and pkn.
In others, the drive for the transferred gene seems to be complementing loss in another compartment.
We made use of this strategy to disrupt the endogenous Pfama1 gene in W2mef while simultaneously complementing loss-of-function with the antigenically distinct 3D7 Pfallelellele (Fig. 1).
Seven days later, perivascular deposition of activated complement components, loss of AQP4 expression, loss of astrocytes and extensive demyelination as well as neuronal cell death were observed.
Interestingly, S. cerevisiae Tor2 can complement the loss of Tor1, but Tor1 cannot complement the loss of Tor2 [ 15, 16].
It has been shown that M. tuberculosis ahpC gene could complement the loss of the same gene in Salmonella typhimurium in which the mutant was hypersensitive to reactive nitrogen intermediates [17].
To complement the loss of function studies with gain of function experiments, we forced the expression of ap, lbe, Kr, and msh pan-mesodermally by using the 24B-Gal4 driner in combination with the corresponding UAS-constructs [36].
To complement the loss of Cdk2ap1 expression in Cdk2ap1 knockout mice, we crossed the heterozygous Cdk2ap1 knockout (Cdk2ap1+/−) mice with the K14-Cdk2ap1 transgeniCdk2ap1+/−+/−:K14-Cdk2ap1) mice that overexpress Cdk2ap1 by using humicekerathat14 proverexpress.
Transforming the mutants with pDD1191, expressing the C. reinhardtii ERG3 ORF, showed enhanced resistance to the drug, demonstrating that the algal gene could complement the loss of the yeast ERG3 (Fig. 6B).
To address the question whether the human wild-type and the pathogenic TPI variants may complement the loss of yeast Tpi1 protein, we transformed this strain with centromeric plasmids for the expression of human wild-type or six representative pathogenic TPI variants as indicated.
