Sentence examples for complement is added from inspiring English sources

The sensitivity is limited because the patient blood is often complement-depleted, and, in more sensitive modifications of the test, complement is added and/or papain pretreated RBCs are used [ 79].

Another interesting finding generated by this study was that when IgA1 was co-deposited with IgG1 antibodies, and serum complement was added, the IgG1 antibodies tended to remain on the antigenic surface.

12.5 μL of E. coli CVCC 1515 cells (5 6 × 103 CFU/mL), 50 μL of serial twofold mouse serum, and 25 μL of baby rabbit complement were added into each well of a 96-well cell culture plate.

For HSV-1, the virus was removed at 48 h after inoculation by washing and new culture medium supplemented with monoclonal antibodies to HSV-1 gD and guinea pig complement was added.

After the addition of the patient serum (1 20) and washing, fresh human complement was added at 37°C for 1 h.

Following incubation baby rabbit complement was added to a final volume of 20% and left for 1 h at 37°C.

The cultures were incubated for 2 hr to allow for cell attachment, after which 0.2%CD599 antiserum and 1.5% (vol/vol) freshly thawed complement were added to each dish.

Triton X-100 (1%) and heat-inactivated complement were added to the wells to measure background or maximal absorbance of WST-1, respectively.

To ensure that read orientation did not affect the taxonomic prediction, oligonucleotide counts of the reverse complement were added to every metagenomic and reference signature.

For this purpose, human serum AB (used as source of complement) was added to the medium with antibodies, but it did not increase cell death induction.

The purified protein was concentrated to achieve a final concentration of 100 150 μM in a 300 μl total volume, and the incorporation of the spin label was confirmed by MS. For experiments in the presence of DNA, a 16 bp duplex assembled from the oligonucleotide 5′-CCCGGCGTGCGGCCCG-3′ and its complement, was added to the protein sample to a final concentration of 100 μM.