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The high frequency of MBL deficiency and functional defects [5], [9] makes MBL mediated complement function assays unsuitable for elucidation of deficiencies downstream of MBL itself.
An overview of complement function is provided in order to understand the underlying genomic landscape.
To exclude that complement activity played a role, whole blood circulation experiments were also conducted at 10 U/ml of soluble heparin, a concentration which inhibited complement function.
Petry, F., Botto, M., Holtappels, R., Walport, M. J. & Loos, M. Reconstitution of the complement function in C1q-deficient (C1qa−/−) mice with wild-type bone marrow cells.
To preserve complement function while prevent clotting, blood from healthy donors was treated with a low dose (1 U/ml) of soluble heparin and was kept in rotation in a Corline heparin inner surface tubing system.
The jawless fishes have complement components corresponding only to the later-acting (i.e., cytolytic, or cell-killing) aspects of complement function, but all higher vertebrates have components similar to the complete complement system of mammals.
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The functional diversity provided by disordered regions is believed to complement functions of ordered protein regions by protein-protein interactions [41].
Complement functions are similar among many species, and corresponding components from one species can carry out the same functions when introduced into another species.
In addition to participating in immune and inflammatory responses and host resistance, there is increasing evidence that complement functions in tissue remodeling and organ regeneration (Mastellos and Lambris 2002).
As the downstream protein of MBL, improper MASP activity can interfere with complement functions [ 24]. there is an association between the genetics of MASP2 and its serum level [ 24- 27].
A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14.
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