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All dogs were negative for Leishmania by direct immunofluorescence (RIFI) and complement fixation tests (RFC).
Complement fixation tests against ZIKV and YFV antigens confirmed these interpretations.
The virus was quickly recognized as an influenza A virus by complement fixation tests.
Complement fixation tests for phase 1 and phase 2 antibodies were performed at the Public Health Laboratory Service, Cardiff.
Complement fixation tests, numbers refer to reciprocal of serum titer/reciprocal of antigen titer; 0, negative reaction at <8/<8.
All dogs were positive for Leishmania by indirect immunofluorescence (IIF), complement fixation tests (CFT) and ELISA (enzyme-linked immunosorbent assay).
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Each blood serum was subjected to both complement fixation test (CFT) and indirect ELISA (I-ELISA; IDEXX Brucella ovis Ab Test; Idexx Montpellier, France) as described by Praud et al. [ 8].
To date, evidence for ZIKV in wildlife in South Asia is limited to serological detection (complement fixation test) in three rodent species, the bandicoot rat (Bandicota bengalensis), Indian gerbil (Tatera indica), and Indian desert gerbil (Meriones hurrianae 33.
Antibody response was assessed by classical tests (Rose Bengal test, complement fixation test (CFT)) in comparison with other diagnostic tests (indirect ELISA (iELISA), competitive ELISA (cELISA), fluorescence polarization assay (FPA), immunocapture test (ICT)).
A panel of 180 horse field sera, previously sent for testing to the German National Reference Laboratory for Dourine, was tested by complement fixation test using culture-derived as well as conventionally produced dourine antigen.
Therefore, the difference in the capsid region is one of the useful markers in the genetic classification between Sagiyama virus and strains of Getah virus, and might be responsible for the serological difference in complement fixation test.
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