Each blood serum was subjected to both complement fixation test (CFT) and indirect ELISA (I-ELISA; IDEXX Brucella ovis Ab Test; Idexx Montpellier, France) as described by Praud et al. [ 8].
To date, evidence for ZIKV in wildlife in South Asia is limited to serological detection (complement fixation test) in three rodent species, the bandicoot rat (Bandicota bengalensis), Indian gerbil (Tatera indica), and Indian desert gerbil (Meriones hurrianae 33.
Therefore, the difference in the capsid region is one of the useful markers in the genetic classification between Sagiyama virus and strains of Getah virus, and might be responsible for the serological difference in complement fixation test.
A panel of 180 horse field sera, previously sent for testing to the German National Reference Laboratory for Dourine, was tested by complement fixation test using culture-derived as well as conventionally produced dourine antigen.
Antibody response was assessed by classical tests (Rose Bengal test, complement fixation test (CFT)) in comparison with other diagnostic tests (indirect ELISA (iELISA), competitive ELISA (cELISA), fluorescence polarization assay (FPA), immunocapture test (ICT)).
In its toxic phase, the disease was easily confused with typhus, so Fairley developed a complement fixation test for the disease along the lines of the Wassermann test.
The principal methods of diagnosis of bovine brucellosis are culture, the complement fixation test, serum agglutination test, Rose-Bengal test (RBT), indirect enzyme-linked immunosorbent assay and more recently the competitive ELISA (cELISA) and the fluorescent polarisation assay.
However, immunodiffusion and complement fixation test results suggest that the NA OPXV are most closely allied to the traditional members (Vaccinia virus, Variola virus and Cowpox virus) of the OPXV.
In 1972, the cattle prevalence was 16.3% in Ankole (southwest), 12.4% in Karamoja (northeast), 19.7% in West Nile (northwest) and 18.7% in Tororo (southeast) using the complement fixation test [3].
Serological techniques for identifying tsetse fly bloodmeals, including precipitin and haemagglutination test [11], complement fixation test [12] and enzyme-linked immunosorbent assays (ELISA) [13], [14] have been applied with reasonable success.
Moose sera were analyzed with a complement fixation test (CFT).
