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Mutated primers were: 5′-CCACTGGTCATCCTCCGGAAAAAACGGGGCCA-3′ and its reverse complement (first mutagenesis); and 5′-CACAGCACAATTCCATTAGTCATCCTCCGGAAA-3′ and its reverse complement (second mutagenesis).
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In the following examples each sentence contains four components: subject, verb, and two complements, first and second, or inner and outer.
Mr. Mullen marries pickled anchovies to salted ones on toast kissed with olive oil, with soft sheep's-milk cheese and slow-roasted tomatoes for taste and texture, the fruit complemented first by sherry vinegar and then a drizzle of aged balsamic for sweetness and wealth.
These are expected to substitute for oil, complementing first generation biofuels.
The third thread can complement the first two in various ways.
Starting in 1994, the New York Road Runners Club used a partial lottery in Central Park to complement the first-come-first-served registration for the New York City Marathon.
As fears grow that the economy will slow sharply over coming months, there are expectations the Bank will complement the first rate cut since March 2009 with other measures.
This analysis will complement the first one since it will allow for correcting for confounding factors and analyzing effect modifications.
We let r be the number of residues that can potentially complement the first mutation to provide the full two-residue adaptive feature.
To this end, the melon gene complement was first grouped in families of homologous genes as explained in the Methods section.
For non-primate species, a second search using the sequence of the mouse genomic region containing the Murine orthologous gene would be used as a query, if possible, to complement the first search.
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