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This protection was independent from Ig or complement deposition in the tissues.
Based on the lack of complement deposition in biopsy and negative MXA staining results an autoinflammatory syndrome is favored over one of autoimmune disease.
By preventing complement deposition, it also confers some degree of resistance to phagocytosis [35], [36].
In contrast, bacteria incubated with heat-inactivated NHS did not show evidence of complement deposition.
Finally, the minimal complement deposition observed on untreated BSA in low serum dilutions was eliminated when adding SPS (Data S3).
These results demonstrate that the complement deposition in intermediate serum dilutions is specific for acetylated BSA and not untreated BSA.
In contrast, complement deposition on the ΔB strain was only slightly affected by the addition of Fg.
When adding Ficolin-3 depleted serum to the plates however, the complement deposition was remarkably reduced (Figure 4C).
Evaluating depleted sera showed, that Ficolin-2 does not contribute substantially to complement deposition in this assay.
First, anti-AQP4-IgG1 and complement deposition can be found in CNS lesions of NMO patients [17], [18].
Through binding to serum proteins such as immunoglobulins, fibrinogen, and the complement regulator, factor H, M family proteins can effectively avoid phagocytosis through prevention of complement deposition.
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