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Controls included identical sets of dishes containing antiserum alone, complement alone, or neither agent.
Exposure to baby rabbit complement alone did not produce malformations in the embryo (Fig. 2D and G).
Incubation in baby rabbit complement alone at a dilution of 1 40 did not increase cell death in the epiblast (Fig. 2A).
We calculated mutant fractions as the number of surviving colonies divided by the total number of cells plated after correction for any nonspecific killing due to complement alone.
Mutant fractions were calculated as the number of surviving colonies divided by the total number of cells plated after correction for non-specific killing because of complement alone.
Complement alone may be inadequate to sustain chronic inflammatory responses to immune complex deposition, because stimulatory and inhibitory FcγR may in fact be the primary regulators of subsequent immune responses (for review [ 21]).
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Complement activation alone in MI amplifies damage to the myocardium [ 25].
As a limitation of the study, a direct causal effect of complement activation alone cannot be proven from the present data.
Control experiments were performed to demonstrate the specificity of fluorescence complementation: e.g., YFPN alone was unable to complement YFPC-RGS14 and YFPC alone was unable to complement YFPN-H-Ras(G12S).
This fraction, but not purified recombinant PUF60 alone, complemented splicing when combined with U2AF65/35 in extract depleted of poly(U -binding proteins [16].
Using the single gene complemented lines (+HASPB and +SHERP), we have shown that HASPB alone can complement the null phenotype observed with the KO parasites, with these parasites able to complete classical development and colonize the SV in late-stage infections.
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