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The first type of tree was generated by complement alignment of the sequences using ClustalX [ 73], and computing the pairwise distance-matrix of the aligned sequences with PHYLIP software package [ 74].
Accordingly, target prediction was performed by reverse complement alignment of the miRNA to the CDS of 51,917 genes (predicted from transcriptomic and genomic data, in the following referred to as the genomic gene set) as well as to the 3′ UTRs of 12,858 of these mRNAs.
Ideally, a perfect alignment would result in a continuous diagonal line from the start to the end of genomes; the presence of a chromosome inversion is highlighted by a break in the diagonal, with the change in dots direction indicating an inverse complement alignment.
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This method can thus be explored as a potential tool to complement alignment-based, clustering-based, and structure-based methods for predicting protein function.
The converted reads were aligned to the converted reference by finding all 12-bp perfect matches and then extending to both ends of the treated read, not allowing gaps (reverse complement alignments were not considered).
Appended 'rc' indicates the BAC was reverse complemented before alignment.
It complements sequence alignment information with Interpolated Markov Models (IMMs) based on frequencies of oligonucleotide sequences.
We could also use structural variables to complement sequence alignments in the detection of functional RNAs [ 48].
The BRaliBase alignments were chosen to complement the SILVA alignments with test data from a source not affiliated in any way with the authors of this article.
For each Hirudo transcript, for its complement of aligned proteins, each word from each description was assigned a weight based on sequence alignment quality.
In addition, a portion of the PZA nucleotide sequence was reverse complemented to allow alignment with other phages of the cluster.
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