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Rationalizing the choice of the anticoagulant for our study using a complement activity assay [12], we found that sodium citrate had the lowest impact on the three complement pathways, compared with EDTA, heparin and PPACK (see Supporting Information S1) and was therefore used throughout our study.
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BOP-induced changes in complement function were assessed by complement hemolytic activity assay.
The buffers used in the experiment for the alternative complement activity (ACH50) assay were: gelatin verona1 buffer (GVB), isotonic veronal-buffered saline (pH 7.3) containing 5 mM sodium bartiturate, 446 mM NaCl and 0.1% gelatin; EDTA-GVB, GVB containing 10 mM EDTA; and Mg2+-EGTA-GVB, GVB containing 10 mM Mg2+ and 10 mM EGTA (Sunyer and Tort, 1995).
Crystal structures of Mtb-AnPRT in complex with PRPP and with varying occupancies of the two metal binding sites, complemented by activity assay data, indicate that this type III PRT binds a single metal-coordinated species of PRPP, while an adjacent second metal site can be occupied due to a separate binding event.
Classical pathway complement activity was measured by hemolytic assay.
Pilot studies of complement activity aided in standardizing the assay to assure addition of excess complement.
Complement activity was determined using a hemolytic assay.
Complement activity was determined using a hemolytic assay and joint morphology was assessed by histology.
For example, Wang et al. [16] have recently demonstrated by an in vitro assay system of complement activity that the the protection of early embryos of zebrafish Danio rerio against microbial attack can be attributed to maternal complement components operating via the alternative pathway (AP).
A) Effect of IgG on total complement activity, measured in a hemolytic AP50 assay with rabbit erythrocytes.
The primary objective was to demonstrate the non-inferiority of MenACWY-TT versus MenC-CRM197 in terms of vaccine response to MenC measured with a serum bactericidal activity assay using rabbit complement (rSBA) in children 2 10 years of age.
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