All data were compiled from a minimum of three replicate experiments.
In the compiled peak tables, the minimum intensity value of each individual peak over all the samples was found, and each of those values was divided by three.
Saccharomyces cerevisiae proteins were compiled and average protein ratios (minimum 2 peptides per protein) were calculated using in-house software from the Hanash lab at Fred Hutchinson Cancer Research Center (Seattle).
Specifically, we eliminated variables with > 85% identical values, and those with the most extreme standardized outlier > 7. We log-transformed and truncated all distance variables at 10 km, and computed additional "compiled" distance variables such as minimum distance to major roads and distance to any port.
The minimum number of species or taxa compiled here is 15,988 for the three Canadian oceans.
Data were compiled from hospital admission records from the Basic Minimum Set of Data (BMSD) from January 2003 to December 2006.
Matched peptides were further filtered at the precursor ion mass accuracy level using a 20 ppm cut-off, while protein hits were compiled and filtered using the StatQuest program with a minimum confidence threshold of 99%.
As calibrations, we used multiple mammalian fossil constraints previously compiled for key nodes [ 48], which include "hard" minimum and "soft" maximum constraints, thus making time estimations less sensitive to the parameters of the prior distributions [ 49].
A convenience store that sells basics like milk, vegetables, cereal, bread, eggs, meat and baby food will require a minimum of 29 licenses, according to a list compiled by the Retailers Association of India.
A review of histological sections and compiled literature on taxa represented in the composite phylogeny, revealed a minimum of ten distinct marginal musculature arrangements (Fig. 1).
