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FS performed the PCR, and assisted with compilation of the sequence.
Although many of the discrepancies between the different human genome assemblies are caused by fundamental disparities in the compilation of the sequence information [ 4], it is still evident that the human genome harbors probably significantly more than 40,000 genes and that these genes are not easily identified.
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Editing and compilation of the sequences was done using Sequencher™version 4.1 (Gene Codes Corporation).
A compilation of the sequences of 27 gp38 adhesins (fig. 3) clearly defines both the most and the least conserved segments of the protein.
Additional primers were designed, as needed, for linking plasmid subclones during the compilation of the initial genome sequences, and for sequencing PCR products and plasmids.
The initial sequence pattern was deduced from the compilation of the Zic family sequences [ 15, 70] and subsequently the intervening sequence lengths were modified to the above pattern until no additional sequences appeared in the search.
Interestingly, early compilations of the Cx26 sequence reported a Ser at codon 86 [48], see also: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi val=AAD21314.1), implying that T86S, which maintains intact the H-bonding capabilities, is simply a polymorphism.
part 2 (compilation of trna sequence) of the bayreuth trna database (http://www.uni-bayreuth.de/departments/biochemie/trna; September 2004 edition) contains a total of 571 tRNA sequences for which there is information about base modifications.
Therefore, GOLD includes a very thorough compilation of the corresponding species sequencing centers, the funding agencies, and contact persons.
A compilation of the 5′ end sequences of Escherichia coli mRNAs has shown that most mRNAs have 40 80 untranslated bases, but some are much longer [ 5].
We produced such cis-regulatory descriptors using the compilation of upstream sequence motifs published by Hughes et al. [ 22] in combination with the genome-wide location analysis showing the binding of transcription factors to gene promoters published by Harbison et al. [ 16].
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