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The specificity of the DNA-protein complex was verified by competition with a 100-fold molar excess of unlabelled specific competitor (corresponding to the respective EgCAD2 and EgCCR regulatory fragments, lanes 3 and 9) whereas an unlabelled non-specific DNA (unrelated DNA fragment, lanes 2 and 8) did not affect the formation of the complexes.
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Specificity of binding was determined by the addition of 100-fold molar excess of unlabeled competitor DNA corresponding to Runx target sequence from CyIIIa [ 12].
At high productivity levels and high predation risk, the predator-resistant inferior competitor dominates, corresponding to the aggregation of M. berthae in non-degraded habitat during the rainy season, where it spatially overlapped with the population of M. coquereli.
The corresponding figure for its competitors, like the Vega, was closer to twenty-seven or twenty-eight miles per hour, he said.
The relative abundance of two sequences in each sample was determined from the ratio of the T/C values of the two comparing sequences after reflecting the copy numbers of their corresponding competitor templates in the competitor array used.
"Top" indicates the maximum amount of radioligand binding (without any competitor) whereas the corresponding value is derived from Eq. (2).
Each test male was isolated two days before facing their assigned competitor and a corresponding female.
With this measurement, the absolute quantity of the target DNA can be determined if the amount of the corresponding competitor is known.
Competition with a 100-fold molar excess of the corresponding unlabelled specific competitor DNA fragments blocked the formation of all the complexes (Fig. 4, lanes 3 and 14) whereas a similar amount of an unlabelled non-specific DNA had no effect (Fig. 4, lanes 2 and 13), indicating the specificity of the interactions.
The fitness of the competitor increases suddenly with a corresponding drop of the incumbent fitness.
The IOD values (StAR/StAR competitor) were normalized with the corresponding L19 expression, and the response of hCG on StAR mRNA expression was assessed.
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