Sentence examples for competitor assays from inspiring English sources

Competitor assays were performed as described above but protein amount was doubled and binding reactions were pre-incubated for 10 min with the non-labelled competitor before adding the fluorescent probe.

Cold competitor assays were carried out adding a 100-fold molar excess of homologous unlabeled oligonucleotides of the labeled dsDNA probe.

For competitor assays, 200-fold molar excess of unlabeled, double-stranded probe, corresponding to 4 pmol, was included in EMSA reactions.

To distinguish competitors during competition assays, cells were plated onto TA solid medium (Lenski, 1991).

In competition assays cold competitor was added at the indicated concentrations simultaneously with probe and protein.

In competition assays, unlabeled competitor nucleic acids were preincubated for 10 min with recombinant proteins before addition of labeled RNA.

All the compounds were first screened for anti-proliferative activity, cytotoxicity and ER-α binding affinity by utilizing ER-α dominant T47D BC cell lines, PBMCs and ER-α competitor assay kit.

The binding affinity of calycosin to ER-α and β was evaluated by the commercially available competitor assay (Invitrogen, Carlasbad, CA) according to the manufacturer's instructions.

Therefore, our study indicates that this direct protein ligand binding competitor assay can be used as an effective alternative method in the early screening of PPARγ ligands.

E2 displacement was assessed with the PolarScreen ER Alpha Competitor Assay Green kit (P2698; Life Technologies) as per the instructions of the manufacturer.

To rule out interaction with the HBS, our most active compounds were tested for E2 displacement using the PolarScreen ER Alpha Competitor Assay Green kit.