Sentence examples for competitive templates from inspiring English sources

TS mRNA expression in all cell lines was studied by competitive template PCR (CT-PCR), using competitive templates designed for β-actin and TS dissolved in standardized dilutions (10−12/10−14, 10−13/10−15, 10−14/10−16 MM, respectively).

Competitive templates were dissolved in standardised solutions.

However, the method requires careful design of competitive templates for each gene.

A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.

Competitive templates were produced from a cell line known to contain a considerable amount of TP activity (Colo320TP1).

Competitive templates were designed for β-actin (Rots et al, 2000) and TP, using the primer sets shown in Table 1 > -wrap-foot>.

Syringic acid was selected as competitive template because it belongs to the same group of phenolic acids with GA.

The competitive binding of GA was evaluated with syringic acid as a competitive template.

The native template to competitive template ratio was calculated and the number of mitochondrial or nuclear DNA molecules was estimated according to Willey et al. [ 7].

ND, Not detectable (When 60 molecules of Competitive Template (CT) were added to the reaction, CT was detected but native template was not).

Competitive template reverse transcriptase polymerase chain reaction (RT PCR) assays for dCK mRNA and the housekeeping gene β-actin were performed as described previously (Kroep et al, 2002).