Your English writing platform
Discover LudwigExact(13)
The competitive binding of GA was evaluated with syringic acid as a competitive template.
Syringic acid was selected as competitive template because it belongs to the same group of phenolic acids with GA.
The native template to competitive template ratio was calculated and the number of mitochondrial or nuclear DNA molecules was estimated according to Willey et al. [ 7].
Competitive template reverse transcriptase polymerase chain reaction (RT PCR) assays for dCK mRNA and the housekeeping gene β-actin were performed as described previously (Kroep et al, 2002).
The quantitative RT PCR technique is based on the coamplification of a competitive template (CT) designed specifically for each different target.
ND, Not detectable (When 60 molecules of Competitive Template (CT) were added to the reaction, CT was detected but native template was not).
Similar(47)
Competitive templates were dissolved in standardised solutions.
However, the method requires careful design of competitive templates for each gene.
Competitive templates were produced from a cell line known to contain a considerable amount of TP activity (Colo320TP1).
A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.
Competitive templates were designed for β-actin (Rots et al, 2000) and TP, using the primer sets shown in Table 1 > -wrap-foot>.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com