In addition to this, we propose a competitive assay for the detection of neomycin B using SPR as a transduction technique.
Mitchell et al. [ 74] also developed a microfluidic SPR biosensor system that utilised competitive assay for SC detection.
In a competitive assay for urinary albumin, albumin-Cy5 was used as a tracer to accept energy from the pyridine-based luminescent donor ITC TEKES Eu, giving a rather high detection limit in the mg L−1 range [ 72].
For competitive assays, at least a 200-fold excess of either specific or non-specific non-labeled DNA was added.
Competitive assays provide the 'gold standard' for measuring fitness.
For EMSA competitive assays (Supplementary Fig. S1), 200-fold of either specific or non-specific non-labeled DNA was added to the binding mixture.
For EMSA competitive assays (Additional file 1), 200 fold of either specific or non-specific non-labeled DNA was added to the binding mixture.
For antibody competitive assays, aliquots of HeLa cell lysates containing 40 μg protein were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to PVDF membranes.
For the agonistic assays, higher CALUX values indicate agonistic transactivation, and for the competitive assays, higher values indicate enhanced (synergistically or additively) transactivation of the natural or synthetic ligands and lower values indicate an antagonistic effect on ligand-induced receptor transactivation (Bonefeld-Jorgensen et al. 2006; Kruger et al. 2007; Long et al. 2006).
We performed competitive binding assays for AR using the PolarScreen AR competition assay (PanVera, Madison, WI, USA) according to the manufacturer's instructions, using polarization fluorometry analysis, with excitation at 485 nm and emission at 535 nm, on a GeniosPro instrument (Tecan Trading AG, Männedorf, Switzerland).
