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Competitive assays confirm that TFD-related structures are the main glycan epitope.
Competitive assays were performed by incubating the target MC-LR and the analyte competitor with horseradish peroxidase-labeled monoclonal anti-MC-LR antibodies (HRP-mAb).
The observed trends in microfluidic immunoassay applications closely resemble the trends of general immunoassays, where large molecules are detected principally through a sandwich procedure, while competitive assays are used to detect smaller molecules.
Significantly lower tumour accumulation was observed in competitive assays and DU145 xenografts.
For competitive assays, at least a 200-fold excess of either specific or non-specific non-labeled DNA was added.
Competitive assays were performed in mice by intravenously inoculating them with bacteria to examine the in vivo fitness of the Δhfq::KmR mutant compared to WT strain 201.
Similar(27)
This immunosensor was employed in a competitive assay involving progesterone labelled with alkaline phosphatase.
The competitive assay is sensitive and selective to Aβ peptides.
These epitope maps are also consistent with results from a competitive assay (Supplementary Fig. 9d).
The possibility of using these particles as agents for DNA sensing was demonstrated in a competitive assay.
According to immunochemistry theory, a common and apt protocol for SMCs is the competitive assay.
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