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In addition to this, we propose a competitive assay for the detection of neomycin B using SPR as a transduction technique.
Mitchell et al. [ 74] also developed a microfluidic SPR biosensor system that utilised competitive assay for SC detection.
In a competitive assay for urinary albumin, albumin-Cy5 was used as a tracer to accept energy from the pyridine-based luminescent donor ITC TEKES Eu, giving a rather high detection limit in the mg L−1 range [ 72].
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Two competitive assays for serum T4 have also been developed using T4 BSA BTBCT23 or T4 BSA biotin/streptavidin BCTOT as competing analytes to give low nmol L−1 detection limits, which is sufficient for clinical diagnosis [ 40, 42].
A competitive assay is well-suited for food analysis and monitoring of gluten digestion because it can detect both intact proteins and small protein fragments, the latter of which can be underestimated by a sandwich ELISA [32], [35].
The MB DNA biosensor has been used directly to detect, in real time, its target DNA molecules without the need for a competitive assay.
For ATP competitive assay, conditions were the same as above except that varying concentrations of ATP were added in the absence or presence of a single concentration of MdOS to generate ATP concentration curves.
The α D-CTX ELISA is a competitive assay based on polyclonal rabbit antibodies for the α D form of CTX.
Peptides were assayed for binding affinity using a filtration-based competitive assay to sstr2.
The specificity for TfR is demonstrated by competitive assay using TfR antibody and siRNA.
The possibility of using these particles as agents for DNA sensing was demonstrated in a competitive assay.
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